LOCUS       40924_48608        4459 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZT90 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Satoh K, Tu Z, Ohba H, Narumi I.
  TITLE     Development of versatile shuttle vectors for Deinococcus grandis
  JOURNAL   Plasmid 62 (1), 1-9 (2009)
  PUBMED    19504653
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Narumi I.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2008) Contact:Issay Narumi Japan Atomic Energy 
            Agency, Quantum Beam Science Directorate; 1233 Watanuki, Takasaki, 
            Gunma 370-1292, Japan
REFERENCE   3  (bases 1 to 4459)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4459)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2009"; volume: "62"; issue: "1"; pages: "1-9"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2008) Contact:Issay Narumi Japan Atomic Energy Agency, 
            Quantum Beam Science Directorate; 1233 Watanuki, Takasaki, Gunma 
            370-1292, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             205..1710
                     /codon_start=1
                     /gene="orf2"
                     /label=orf2
                     /note="Orf2; similar to Deinococcus radiodurans plasmid
                     pUE10 RepU"
                     /protein_id="BAH03366.1"
                     /translation="MAQKISREGGAGPAVHPNWQHPAGRRGAKVILEASQKCLQTPLIE
                     PSHSEGPLPMNNQKQDSYTDRVRQIAPVVPLLDLIVKPLIPERAAPNPYLEMGLAALRG
                     EPVDTRRAQVQPSKQFQKASRPAPAAPPLVLTSDQGEDQEAAMLLPEIVEETRRRLEKQ
                     ARENRETRRAEQRARFHQLVTAPTDEAAPDFVPPVSLQEGRQPPSGSVRVTLPRPEVPG
                     PAWGELSADAVLDSVPWLSKGARLVFRIMHMLAVAKAQDCRYPVIPNSAAFHTPQLLLA
                     FVARYTTRHFRRLTNELEHAGVIDGGAHAAKVKTGIGTTRHLWDGSIWAVKLRPSTCDA
                     YLSPEDWQHEWRDFQADLESGRTAKKLMSYLNTCEGLARQEHVLKTWAVNPNAKMTSLC
                     LERTFQAEQKMTLQDTVYALPLIGELSELKQAGAIGNAASIISHALGDSHSRRFWCGLL
                     WAATRNGSLEAFSAQLLRLLADVQESSELRNPGALFAARLRSA"
     gene            205..1710
                     /gene="orf2"
                     /label=orf2
     misc_feature    1778..2060
                     /note="region of low %G+C (36%); possibly involved in 
                     replication initiation"
     primer_bind     complement(2123..2139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2356..2944
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3232..3253
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3268..3298
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3306..3322
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3330..3346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3498..4154)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      complement(4212..4240)
                     /label=derived from Deinococcus radiodurans katA promoter
                     /note="derived from Deinococcus radiodurans katA promoter"
                     /regulatory_class="promoter"
     misc_feature    4288..4293
                     /label=EcoT22I site
                     /note="EcoT22I site"
     regulatory      complement(4379..4408)
                     /label=derived from Deinococcus radiodurans groE promoter
                     /note="derived from Deinococcus radiodurans groE promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(4427..4443)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cccaaatcct gaccgtgcca aagccccagc aaaaacagga attgggtgcc gattttctgc
       61 gggtgctggg gtgttacgca agattagcac taaagtgcat cttgcctctg aaactgcgtt
      121 ccagagggga aaaaaccagg gtgctgttaa ttttaattac tggtcaactt ttgggccgtt
      181 tttcgagggg tcaatgcgcg agaaatggcc caaaaaatca gcagggaggg aggtgctgga
      241 cctgccgttc accccaactg gcaacacccc gctgggaggc ggggtgctaa ggtaattctc
      301 gaagccagtc aaaagtgtct ccaaacaccg ttgattgagc catcccactc cgaaggacca
      361 cttccgatga acaatcaaaa gcaggacagc tataccgata gggtacggca aatcgccccc
      421 gtagttccac tcctggacct cattgttaag cccctcatcc ctgaacgagc agcccctaat
      481 ccctatctgg agatgggcct agccgccctg cgtggcgagc cggtggacac ccgccgcgcc
      541 caggtgcagc ccagcaagca gttccagaag gccagccgcc cagccccagc cgcccccccc
      601 cttgtgctga cctcagacca gggcgaggac caggaggccg ccatgctgct gcccgagatc
      661 gtcgaagaga cgcgccgccg cctggagaag caggcccgcg agaaccgcga gacgcgccgc
      721 gccgaacagc gcgcccgctt tcaccagctc gtgaccgccc ccaccgacga ggccgcgccg
      781 gacttcgtgc cgcctgtgag ccttcaggaa ggccgtcagc cccccagcgg cagtgtgcgc
      841 gtcaccttgc cccgccctga agtccctggg cctgcctggg gcgagctgag cgccgacgcg
      901 gtgcttgatt cggtgccgtg gctgagcaag ggcgcgcggc tggtgttccg catcatgcac
      961 atgctggctg tggcgaaggc ccaggactgc cggtatccgg tgatccccaa ctcggcggcc
     1021 ttccacacgc cccaactgtt gctggccttc gtggcgcggt acaccacccg gcacttccgg
     1081 cgactgacca atgagctgga gcacgctggg gtgattgacg gcggcgcgca cgccgcgaag
     1141 gtcaagaccg gcatcggcac cacccggcac ctgtgggacg gctcgatctg ggcggtcaag
     1201 ctacggccca gcacctgtga cgcctacctg tcccccgagg actggcagca cgagtggcga
     1261 gacttccagg cggacttgga gtcgggcagg acggcgaaaa agctaatgtc ctatctaaac
     1321 acctgtgagg gtttagccag gcaggagcac gtcctgaaaa cctgggccgt taatcccaat
     1381 gcgaaaatga cttcgttgtg tttagagcgg acatttcagg ccgagcagaa aatgaccctc
     1441 caggacacgg tttatgccct gccgctgatc ggggaactga gcgaactcaa gcaggccgga
     1501 gccatcggaa acgcggcgtc gatcatctcg cacgccctgg gcgacagcca cagccgaaga
     1561 ttctggtgtg ggttgctgtg ggcagccacc cggaacggct ctttagaggc gttctcggcc
     1621 cagttgctgc ggttgctggc ggatgtgcag gagtccagcg agctgagaaa ccccggcgcg
     1681 ctgttcgcgg cccgccttcg cagcgcctga ccttcgggga ccgcgcccga agaccaggcg
     1741 ctgctcagtt gggcttgagg gtgattacgt tctgagcata tgaccgcatg aggacatctg
     1801 ttaatagcgt aaaaatctct ctgctctaat gacatttagg gcagagaaac tatagaaaac
     1861 ttattcggtt tttcttgcag aagagattag aactagtaat cagttctatt tttttattaa
     1921 acagatttat tctctcaact attataattc ctataccgaa gagtacgaag atggatgcca
     1981 agagcatagt cattactccc ttttgcattg tcgctccttt caaatcgtat gatacagtgt
     2041 tctgacgcgg gtaaaatttt ggtttcacca ctcacgcact tttggaggcc cgatcatgtc
     2101 tgagggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2161 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2221 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2281 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2341 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2401 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2461 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2521 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2581 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2641 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2701 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2761 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2821 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2881 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2941 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3001 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3061 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     3241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     3301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     3361 gcttgcatgc ctgcaggtcg actctagagg atcctctaga gcttcgacga atttctgcca
     3421 ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa
     3481 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     3541 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     3601 atcagcacct tgtcgccttg cgtataatat ttgcccatcg tgaaaacggg ggcgaagaag
     3661 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     3721 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     3781 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     3841 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     3901 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     3961 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     4021 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     4081 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     4141 atttttttct ccatatgctc tccttcgcct cgctggctct gctgcgcggc aagaggcccg
     4201 aagctccctg caccatattg agaatcattc tcaatgtcaa gggccctcgg tctccatgcg
     4261 agctcgaatt cgatatcaag cttatcgatg catgtggggt cctcctgtga gtgagatgga
     4321 atggcggaga gtcgtgccgg cgagcttttt cccgcccagc gccttcaatc cgtcacggat
     4381 ggtagagcgc cgataagaaa aatgtcaact gaccgaagct gacaatcgat accgtcgacc
     4441 tcgagggggg gcccggtac
//
